Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
Blog Article
Information-enriched characterization of mobile populations. Morphological information from visuals can incorporate into the richness of flow cytometry knowledge. By way of example, the figure reveals an or else regular apoptosis assay applying Annexin V and PI, including mobile imaging to characterize cells in each populace to reveal morphologically distinctive functions. These insights could not have been gained from multiplex staining on your own.
As samples are obtained within the Attune CytPix Flow Cytometer, the substantial-velocity brightfield digital camera captures and retailers photographs of detected functions in a charge nearly six,000 illustrations or photos for every second, with regards to the flow price and image sizing. For increased flexibility, Attune Cytometric Application helps you to modify the graphic capture frequency as essential and choose a certain gate for impression seize, if necessary.
(B) Uncooked 264.seven macrophages are frequently annotated inaccurately with respect to particle count due to their complexity and noncircular borders. The volume of singlets accessible for Assessment will increase when the user trains and employs a far more precise model.
Explore new insights into your experimental data by visualizing populations making use of combined fluorescence and morphology info. Our application interface wizards allow downsampling, concatenation (merged documents) and dimensionality instruments that happen to be constructed into the software for an intuitive, streamlined information analysis practical experience.
"A picture isn't really really worth a thousand dots, but it surely really really helps to see photos. It is excellent to view pictures that correlate with staining. One example is, AnnexinV-optimistic cells that are morphologically various than healthful cells, or CD14+ monocytes that happen to be bigger and even more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a highly trained user has gated singlets confidently. Just after analyzing the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate has greater than four% aggregates.
Pair your Attune flow cytometer having an autosampler for amplified workflow efficiency, and incorporate robotic integration for maximum throughput.
Constant image quality even at substantial throughput Acoustic concentrating plus a higher-velocity digital camera Incorporate to impression these T cells constantly at minimal or significant flow fees. Quickly alter focus and camera configurations to satisfy experimental needs.
The Attune NxT cytometer would be the reputable workhorse instrument your lab can depend upon for regular and trusted functionality. The Attune CytPix design incorporates a higher-speed brightfield digicam and AI-driven impression analysis software program, with new updates such as device Finding out applications and an intuitive user interface, enabling Improved data precision, precision, and more quickly insights into sample biology.
Potentially most importantly, these gatherings comprise cells of Evidently different phenotypes which may lead to check here incorrect conclusions about double optimistic activities (particularly in exceptional populations).
AI-pushed automatic impression Evaluation Together with the Attune Cytometric Application translates party options into unique morphology parameters which can be combined with regular fluorescence and scatter parameters to reveal very reproducible population modifications and exceptional subsets.
Software Be aware: Automatic image Evaluation decreases person-to-consumer variability in flow cytometry gating techniques
Leverage the power of State-of-the-art engineering and innovation to accelerate your discoveries. Have confidence in inside the Attune Flow Cytometer loved ones to deliver the reliability, speed, and accuracy that established your get the job done apart.
No clean/no lyse leukocyte Investigation with imaging. Cells had been obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Skilled UX/UI permits consumers to practice AI products for expanded programs. (A) The “Coach Design” function offers annotation resources and genuine-time product instruction accuracy.
At the center of each and every Attune Flow Cytometer lies our revolutionary acoustic concentrating technological innovation, which utilizes audio waves to meticulously align cells inside the flow mobile. This modern technique surpasses traditional hydrodynamic procedures, causing quicker processing speeds, Extraordinary details high-quality, and amplified sample throughput.
As the mobile line experienced Beforehand been analyzed about the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and were being in the position to doc the microbial infection a minimum of five days earlier.
Agilent Price Promise guarantees you at least ten years of instrument use in the day you purchase. Otherwise, We're going to credit you Using the residual price of the procedure toward an upgraded product.
Below, we demonstrate that by gating techniques utilizing impression-based mostly quantitative parameters can distinguish interacting cells from coincident functions to much more properly analyze interacting cells.